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. Author manuscript; available in PMC: 2016 Apr 6.
Published in final edited form as: J Bone Miner Res. 2015 Oct 13;31(3):606–614. doi: 10.1002/jbmr.2723

Fig. 5.

Fig. 5

ERβ knockdown enhances ERα-specific transcriptional activity. MC3T3-E1 cells were cotransfected with either a negative control or ERβ-specific siRNA and an ERE-luciferase reporter construct. (A) QPCR analyses of ERβ expression in both the negative control and ERβ-specific siRNA-transfected cells to account for knockdown efficiency (n = 3). The asterisk represents p <0.01 compared with the negative control siRNA. (B) A parallel set of identically transfected cells were treated with either vehicle control or the ERα-specific agonist PPT (10 nM). Twenty-four hours later, the cells were harvested and luciferase and protein assays were performed. The data are expressed as luciferase activity/μg protein, graphed relative to the vehicle control condition for each siRNA and represent mean ± SD (n = 6). A single asterisk (*) represents p <0.01 compared with the vehicle control for each siRNA condition. A double asterisk (**) represents p <0.01 comparing the PPT-treated cells between the negative control and ERβ-specific siRNA conditions.