Table 2.
Alterations in Prespecified Gene Pathways in the ERβPrx1–CKO Versus Control Micea
| Pathway | Direction of regulation | Genes | p Value |
|---|---|---|---|
| Autophagy | ↓ | atg5 (0.69*), atg7 (0.99), map1lc3a (0.66*), nbr1 (0.87*), | 0.001 |
| Proliferation | ↓ | ccna2 (0.88), ccnb1 (0.76*), ccnb2 (0.91), ccnc (0.81*), ccnd1 (0.66*), ccnd2 (0.92), ccne1 (0.92), e2f1 (0.67*), mki67 (0.77*) | 0.001 |
| Adipogenesis | ↓ | adipoq (0.67*), cebpa (0.75*), cebpb (0.82), cebpd (0.94), cfd (0.50*), lep (0.34*), lpl (0.69*), plin1 (0.48*), pparg (0.83) | 0.004 |
| Senescence | ↓ | bax (0.81), cdkn1a (0.99), cdkn2a (1.15), cdkn2d (0.53*), foxo3 (0.89), igfbp2 (0.97), pten (0.89), rb1 (0.94), sirt1 (0.94), tp53 (0.60*) | 0.009 |
| Circadian rhythm | ↓ | arntl (0.94), clock (0.75*), cry1 (0.89), cry2 (0.70*), csnk1e (0.88), per1 (0.82), per2 (0.94), per3 (0.86) | 0.014 |
| Wnt targets | ↓ | axin2 (1.08), birc5 (0.67*), ccnd1 (0.66), cyr61 (0.83), ephb4 (0.97), fosl1 (1.01), gja1 (0.94), lef1 (1.06), postn (0.70), tcf7 (0.92), tnfrsf11b (0.78), vcan (0.82) | 0.019 |
| β-Adrenergic targets | ↓ | ccnd1 (0.66), ccnd2 (0.92), ccne1 (0.92), cry1 (0.89), cry2 (0.70), myc (0.72*), per1 (0.82) | 0.002 |
| Oxidative stress | ↓ | cat (0.81*), foxo1 (1.00), foxo3 (0.89), foxo4 (0.86), gadd45a (0.72*), sod1 (0.79*), sod2 (0.90), sod3 (0.77) | 0.002 |
| Apoptosis | ↓ | bad (0.88), bax (0.81*), bcl2 (0.82*), bcl2l1 (0.79*), casp3 (0.92), casp8 (0.91), fas (0.85), faslg (0.98) | 0.002 |
| Adhesion | ↓ | alcam (0.80*), cdh2 (0.76*), icam1 (0.74*), vcam1(1.25) | 0.003 |
| Inflammation | ↓ | csf1 (0.87), ifng (0.73), il1a (1.02), il1b (1.30), il6 (0.84), nfkb1 (0.85*), nfkb2 (0.98), nfkbia (1.15), relb (0.95), tnf (0.72*) | 0.043 |
| Osteoblast differentiation | alpl, bgalp, col1a1, col1a2, ibsp, runx2, runx2p1, runx2p2, sp7, sparc, spp1 | 0.060 | |
| Notch | hes1, hey1, heyl, jag1, jag2, notch1, notch 2 | 0.139 | |
| BMP targets | hes1, id1, id2, junb, klf10, lox, smad6, smad7, sox4, zeb1 | 0.188 | |
| Osteocyte | dmp1, fgf23, mepe, pdpn, phex, sost | 0.216 | |
| Stem cell markers | nanog, nes, pou5f1, sox2, tert | 0.426 |
a
Shown is the list of genes examined in each of the pathways analyzed along with the results of the cluster analyses testing for changes in the genes in each pathway as a group, using the Fisher’s test (see Statistical Analyses). The p values and pathways differing between the 2 groups are highlighted in bold and the numbers in parentheses for the genes in the significant pathways indicate fold changes in the ERβPrx1–CKO relative to the control mice. Asterisks represent p <0.05 for that gene.