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. Author manuscript; available in PMC: 2016 Apr 14.
Published in final edited form as: Cell Rep. 2015 Apr 14;11(2):183–191. doi: 10.1016/j.celrep.2015.03.025

Figure 1. Hippocampal-specific ATF4 down-regulation.

Figure 1

(A) Representative western blot showing ATF4 protein knockdown in primary hippocampal neuronal cultures infected at 5 DIV for 2 weeks with shATF4-lentivirus compared to shCTRL2-infected and non-infected cultures.

(B) Distribution and expression of GFP in mouse hippocampus 1 month after infection with GFP-expressing lentivirus. Sections of lentivirus-injected animals were immunolabeled with antibody against GFP (green) and counterstained with Topro3 (nuclei, blue). Scale bar 20μm.

(C) RT-qPCR analysis of ATF4 mRNA levels in mouse hippocampus 4 weeks after lenti-shATF4 injection. Data are expressed as mean ± SEM (shCTRL2 n=7, shATF4 n=7) (*p<0.05).

(D) Scheme of experimental design: 3-month-old hippocampi of C57BL/6 wt male mice were stereotaxically inoculated either with lenti-shCTRL2, -shCTRL1, or -shATF4. 4 weeks after injection, cognitive functions of the animals were assessed with a battery of behavioral tests, at the end of which they were sacrificed for electrophysiological analyses.