Fig. 3.

Procedure for selecting transcription factors for further analysis. (A) Scatterplot of susceptibility signature vs. promoter affinity scores for Gcn4p. (B) Pearson correlation (t value) between the susceptibility signature of the seven selected TFs and the promoter affinity scores of all 123 TFs. A red dot denotes the same TF and a gray dot other TFs. The arrow points to the t value of the correlation depicted in A. We accepted the TFs whose susceptibility was most strongly correlated to promoter affinity computed using the PSAM for the same TF. The blue line represents a 1% FDR threshold (t = 4.22, P = 3.9 × 10⁻⁶). Susceptibilities were obtained by performing multiple regression of the mRNA expression level of each gene on the activities of all TFs.