Fig. 8.

Validation of the prediction that DIG2 is a cQTL that modulates the activity of Ste12p. The transcriptional response to mating pheromone, in different genetic backgrounds, for a reporter driven by a promoter containing three PREs bound by Ste12p is shown. (A) In the RM background, activation of Ste12p by alpha-factor is robust, but when its DIG2 allele is replaced by that of BY the response is noticeably absent. (B) The BY strain shows a greater response, a fourfold induction that is steady across time. When the DIG2 allele from RM is substituted in BY, the degree of induction is even stronger. All P values were calculated using a two-sample t test on three replicates per strain.