Table 1.
Summary of thiocillin variants produced in this study and their antibiotic efficacy against B. subtilis 168
| Mutant | Compounds observed* | Yield, μg/L† | MIC, μg/mL‡ |
| T3BocK | 8/8 | 686 | 1.5 |
| T3ProcK | 8/8 | 943 | 3 |
| T3AlocK | 4/8 | 713 | 1.5 |
| T4BocK | 0/8 | n.d. | n.d. |
| T4ProcK | 2/8 | n.d. | n.d. |
| T4AlocK | 2/8 | n.d. | n.d. |
| V6BocK | 1/4 | n.d. | n.d. |
| V6ProcK | 2/4 | n.d. | n.d. |
| V6AlocK | 0/4 | n.d. | n.d. |
| T8BocK | 2/4 | 180 | 0.75 |
| T8ProcK | 2/4 | 436 | 1.5 |
| T8AlocK | 4/4 | 667 | 1.5 |
| T13BocK | 2/8 | 125 | >4 |
| T13ProcK | 3/8 | 623 | >4 |
| T13AlocK | 5/8 | 889 | >4 |
| Wild type | 8/8 | 11,200 | 0.38 |
n.d., not determined.
*
Details of expression and analysis can be found in SI Appendix, Table S1.
†
Representative yield after purification of the most abundant compounds.
‡
Minimum inhibitory concentrations were determined by standard dilution assay; experiments were performed in triplicate.