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B. fragilis strains carrying partial T6SS loci are rare. The presence of homologs of BF9343_1943-18 (excluding bte1/bti1 and bte2/bti2ab) in each of 92 sequenced B. fragilis genomes was determined using an E-value cutoff of 0.0001, a percent identity cutoff of 50, and a percent coverage cutoff of 50 (SI Materials and Methods). The paucity of genomes carrying partial T6SSs suggests that once an essential T6SS gene is mutated or lost, the rest of the locus is no longer maintained.
