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. 2016 Mar 14;113(13):3609–3614. doi: 10.1073/pnas.1523686113

Fig. 7.

Fig. 7.

Comparison of the effect of SPR treatment to SM knockout on EBV gene expression in epithelial and lymphoid cells. (A) SMKO EBV-infected 293 cells were transfected with ZTA to induce replication or transfected with both ZTA and SM to rescue SM-dependent gene expression. RNA was prepared from cells 48 h postreplication induction. High-throughput RNA sequencing was performed to determine the effects of SPR or SM knockout on the lytic EBV transcriptome. Differential expression of SM-dependent genes is shown as the ratio of RNA expression in SMKO EBV transfected with Z+SM versus SMKO Z alone. Genes whose expression is most highly dependent on SM are marked in blue. (B) Epithelial AGSiZ cells were treated with doxycycline to induce replication. The differential expression of SPR-sensitive genes during EBV replication is shown as the ratio of RNA in vehicle-treated (–S) versus that in SPR-treated cells (+S). The most SPR-sensitive genes therefore have the highest ratios of RNAs. (C) Comparison of SPR-treated versus untreated EBV-infected P3HR1-ZHT lymphoma cells was performed as in B except that cells were treated with tamoxifen to induce lytic replication. +, values greater than limit of y axis; *, genes in the region that were deleted in B95.8 and P3HR1 genomes.