Fig. S8.

29ddC/62 is an efficient trap. Primer extension was monitored as depicted in Fig. 2. (A) 16% denaturing sequencing gel of the primer extension products for pol δ alone (lanes 1–13) and a pol δ holoenzyme (lanes 14–26) in the presence of 50 µM 29ddC/62 DNA trap. The size of the substrate and full-length product are indicated on the left. Primer extension is not observed for polδ alone (lanes 1–13) over the entire time course. (B) Quantification of the primer-extension products (total, ●) for holoenzyme formed with PCNA. The amount of primer extension products remains flat over the entire time course. Together, this demonstrates the efficiency of the 29ddC/62 DNA to trap any pol δ introduced into solution during an extended incubation.