Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
NMR analysis of N15 labeled ubiquitin in the absence and presence of unlabeled Skp1–F-boxFbw7 protein. Heteronuclear single quantum correlation spectra of 150 µM N15-labeled Ub (red), 150 µM N15-labeled Ub + 240 µM unlabeled Skp1–F-boxFbw7 added (black), and overlay of the two spectra. Absence of chemical shift changes in Ub N15 spectra upon addition of Skp1–F-boxFbw7 indicates no interaction in the micromolar range.
