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. 2016 Mar 16;113(13):E1907–E1916. doi: 10.1073/pnas.1523168113

Fig. 1.

Fig. 1.

Viral SPT is highly expressed at early stages of infection. (A and B) E. huxleyi cultures were infected with EhV201 lytic virus and compared with noninfected control cells. Host cell abundance (A) and extracellular viral production (B) are shown. (C and D) Temporal expression patterns analyzed by qRT-PCR during EhV infection of the viral gene vSPT (C) or the host E. huxleyi-encoded hSPT (D). (E) Western blot analysis using vSPT antibody against protein extracts from control or throughout the course of viral infection. M, protein ladder. (F and G) SPT enzymatic activity assay performed in vitro using three different fatty acyl-CoAs (C14-CoA, C15-CoA, and C16-CoA) and radiolabeled Ser as substrates of protein extracts from control (F) or viral-infected cultures (G). Results are the average ± SD of two biological replicates of one experiment. A similar trend was observed in two additional experiments with similar settings.