Skip to main content
. 2016 Mar 14;113(13):3681–3686. doi: 10.1073/pnas.1523787113

Fig. 4.

Fig. 4.

CYP76AK6 and its homolog, CYP76AK8, catalyze successive oxidation events at C-20. Formation of 1 by CYP76AK6 (pink) and CYP76AK8 (teal) in yeast cells coexpressing CYP76AH24 and SpMilS. TMS-derivatized extracts of yeast cultures analyzed by GC-MS revealed the formation of 1 (Top), identified by comparison with authentic standard (blue) (Bottom). Enzymatic assays containing microsomal preparations of CYP76AH24 ortholog and CYP76AK6 (pink) or CYP76AK8 (teal), 16 and NADPH revealed the formation of 1 in vitro (Middle).