Table S2.
Overview of the experimental data shown in the SI figures
| Transcription stage | Sample | No. of molecules | FRET efficiency | Relative percentage | ||
| E1 | E2 | A1 | A2 | |||
| RNAP only | RNAP ΔRpo4/7 + ES | 348 | 0.48 ± 0.02 | 0.65 ± 0.02 | 55% | 45% |
| RNAP wt + ES | 551 | 0.51 ± 0.04 | 0.65 ± 0.03 | 51% | 49% | |
| RNAP ΔRpo4/6/7 | 448 | 0.34 ± 0.06 | 0.69 ± 0.02 | 15% | 85% | |
| RNAP ΔRpo6 | 231 | 0.34 ± 0.06 | 0.69 ± 0.03 | 15% | 85% | |
| Initiation complex (heteroduplex DNA) | RNAP ΔRpo4/7 | 943 | 0.40 ± 0.01 | 0.62 | 53% | 47% |
| RNAP ΔRpo4/7 + TFE | 750 | 0.43 ± 0.01 | 0.62 | 58% | 42% | |
| Elongation complex | RNAP ΔRpo4/7 dyes swapped | 915 | 0.51 ± 0.01 | 0.65 ± 0.01 | 72% | 28% |
| RNAP ΔRpo4/7 | 741 | 0.48 ± 0.01 | 0.59 ± 0.01 | 53% | 47% | |
| RNAP wt + long RNA | 745 | 0.49 ± 0.01 | 0.63 ± 0.02 | 70% | 30% | |
| RNAP wt +long RNA / + correct NTP | 256 | 0.49 ± 0.01 | 0.62 | 86% | 14% | |
| RNAP wt (T43) + correct NTP | 259 | 0.49 ± 0.01 | 0.60 ± 0.02 | 80% | 20% | |
The elongation scaffold type used in the measurements is indicated in the table, which shows the number of the RNA in the parentheses. ES, elongation scaffold; TS(Bio), biotinylated template strand.