Table 1.

Changes in the Reactive Oxygen Species Production Under Hypoxia in Different Preparations

Tissue/preparation	Hypoxia	[ROS]i	Technique of detection	References
Isolated rabbit lung	Minutes	↓	Lucigenin	Paky et al. (108)
Isolated rat lungs, pulmonary artery ring, PASMCs	Minutes	↓	Lucigenin, amplex red, dihydroethidium, H2DCF	Archer et al. (6)
Isolated rabbit lung	Minutes	↓	Lucigenin	Weissmann et al. (174)
Calf pulmonary arteries	Minutes	↓	Lucigenin	Mohazzab and Wolin (93)
Rat pulmonary artery	Minutes	↓	Lucigenin, amplex red, H2DCF	Michelakis et al. (89)
Rat pulmonary artery	Weeks	↓	L-012 (luminol analogue) amplex red	Bonnet et al. (19)
Human PASMCs	Hours	↓	Lucigenin, amplexred, dihydroethidium, H2DCF	Mehta et al. (88)
Human PASMCs	Minutes	↓	Dihydroethidium	Wu et al. (176)
Isolated mouse lung	Minutes	↑	Electron spin resonance	Weissmann et al. (173)
Rat pulmonary arteries	Minutes	↑	H2DCF	Jernigan et al. (56, 57)
	Weeks	↑
Rat pulmonary arteries	Hours	↑	Dihydroethidium	Wang et al. (162)
Mouse pulmonary arteries, rabbit PASMCs	Minutes	↑	H2DCF	Paddenberg et al. (107)
Porcine pulmonary arteries	Minutes	↑	Electron paramagnetic resonance, H2DCF, lucigenin	Liu et al. (76)
	Weeks	↑
Calf PASMCs	Minutes	↑	Lucigenin	Marshall et al. (85)
Rat PASMCs	Minutes	↑	H2DCF	Killilea et al. (61)
Rat PASMCs	Minutes	↑	Fluorescence resonance energy transfer probe, H2DCF	Waypa et al. (165, 166)
Mouse PASMCs	Minutes	↑	Cytochrome c reduction assay, H2DCF	Rathore et al. (122, 123)
Mouse PASMCs	Minutes	↑	H2DCF, lucigenin, Redox Sensor Red CC-1	Wang et al. (161)
Human PASMCs	Hours	↑	Dihydroethidium	Wu et al. (176)

Modified from Olschewski and Weir (104).

H₂DCF, dichlorodihydrofluorescein; PASMC, pulmonary artery smooth muscle cell; ROS, reactive oxygen species.