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. Author manuscript; available in PMC: 2016 Oct 15.
Published in final edited form as: J Immunol. 2016 Mar 4;196(8):3354–3363. doi: 10.4049/jimmunol.1502687

Figure 2. An Ad-I8V minigene construct switches a non-inflating response to inflation.

Figure 2

C57BL/6 mice were immunised with either of Ad-LacZ (2×109 iu/mouse), Ad-D8V (1×108 iu/mouse) or Ad-I8V (1×108 iu/mouse) (or left naïve). Tetramer-specific responses were tracked in blood over a time course of day 14, 28, 50, 76 and 98-post immunisation. (A) Shows (left to right) the conventional Ad-LacZ immunisation, with the inflating D8V (blue) and the non-inflating I8V (red) responses; Ad-D8V immunisation; Ad-I8V immunisation. Naïve control responses are undetectable. (B) Representative results for the phenotypic markers (CD44, CD62L, CD127 and CD27) from tetramer-specific responses in blood at day 50-post immunisation in the minigene constructs, compared to naïve CD8+ T cells as well as the full phenotypic data at an early and late time-point. (C) Individual D8V and I8V-specific day 100 responses are shown for each of the constructs in the liver, lung and spleen. (D) IFNγ and TNFα production from peptide stimulated day 75-post immunisation splenocytes, from each of the 3 constructs alongside naïve controls. (n=5/group with results showing the mean ± SEM. All work has been performed at least twice independently showing the same results.)