Table II. Summary of AdHu5 processing constructs.
Vector | Promoter | Insert | P:I | Used at (iu/mouse) |
---|---|---|---|---|
Ad-I8V | HCMV | β-gal497-504 | 17 | 1×108 |
Ad-D8V | HCMV | β-gal96-103 | 23 | 1×108 |
Ad-M45 | HCMV | M45985-993 | 25 | 1×108 |
Ad-7aa-I8V | HCMV | β-gal490-504 | 20 | 1×108 |
Ad-10aa-I8V | HCMV | β-gal487-504 | 60 | 1×108 |
Ad-10aa-I8V-10aa | HCMV | β-gal487-514 | 42 | 1×108 |
Ad-I8V-10aa | HCMV | β-gal497-514 | 28 | 1×108 |
Ad-I8V-D8V (Ad-ICD) |
HCMV | β-gal497-504- GGGCCCGGG – β-gal96-103 |
22 | 1×108 |
Ad-D8V-I8V (Ad-DAI) |
HCMV | β-gal96-103 - GGGCCCGGG – β-gal497-504 |
21 | 1×108 |
AdHu5 replication-deficient vectors used with their varying inserts are shown (all have a human IE CMV promoter). P:I ratios (particle number to infectivity) are shown alongside the viral titre in infectious units for which the virus was immunised into a single mouse.