Figure 5.

In vitro production of different kalata B1 cylotides using an intein-mediated backbone cyclization (R. H. Kimura et al., 2006). A. The intein-kalata constructs were expressed in E. coli, purified and cyclized/folded in aqueous buffer at pH 7.2 containing reduced GSH for 24 h. B. Sequences of all the katala B mutants cyclized using this approach. C. Analytical HPLC traces of the cyclization/folding crude after 18 h. The peak corresponding to the folded cyclotide is marked with an asterisk.