Table 4.
Resistance, ESBL genes and genetic relatedness of pairs of E. coli isolates of fecal origin (dogs and humans within a household) with similar profiles.
| Isolatea | Host species | Resistance phenotypeb | ESBL genes |
Phylogroup | PFGEc | ||
|---|---|---|---|---|---|---|---|
| blaTEM | blaCTX-M | blaSHV | |||||
| 15F | Dog | AMP AMC CFE CAZ CTX ATM | − | + | − | D | − |
| 15H | Human | AMP AMC CFE CTX CRO | − | + | − | A | − |
| 23F | Dog | AMP EST CLO SUT TET | − | − | − | D | I |
| 23H | Human | AMP EST CLO SUT TET | − | − | − | A | I |
| 27F | Dog | AMP CFE EST | − | − | − | B1 | D |
| 27H | Human | AMP CFE EST | − | − | − | A | D |
| 48F | Dog | AMP CFE CAZ CTX CRO GEN EST CLO SUT | + | + | − | A | I |
| 48H | Human | AMP CFE CTX CRO GEN EST CLO SUT TET | + | + | − | B1 | I |
| 49F | Dog | AMP AMC CFE CPM CTX CRO GEN EST CLO CIP SUT TET | − | + | − | B1 | D |
| 49H | Human | AMP CFE CPM CTX CRO GEN EST CLO SUT TET | + | − | − | B1 | D |
| 59F | Dog | AMP EST | − | − | − | B1 | D |
| 59H | Human | AMP EST | − | − | − | B1 | D |
| 61F | Dog | AMP AMC EST CLO SUT TET DOX | − | − | − | B2 | I |
| 61H | Human | AMP AMC EST CLO SUT TET DOX | − | − | − | B2 | I |
| 58F | Dog | AMP AMC EST CLO | + | − | − | B1 | − |
| 58H | Human | AMP EST CLO | − | − | − | B1 | − |
| 92F | Dog | AMP CAZ ATM GEN EST CIP SUT TET DOX | + | + | + | B1 | I |
| 92H | Human | AMP ATM GEN EST CIP SUT TET DOX | + | − | + | B1 | I |
a
The letter F designates canine fecal isolates; H, human fecal isolates (dog owners).
b
AMP, ampicilin; AMC, amoxicillin + clavulanic acid; CFE, cephalexin; CAZ, ceftazidime; CPM, cefepime; CTX, cefotaxime; CRO, ceftriaxone; ATM, aztreonam; GEN, gentamicin; EST, streptomycin; CLO, chloramphenicol; CIP, ciprofloxacin; SUT, sulfametoxazol + trimethoprim; TET, tetracycline; DOX, doxycycline.
c
Tenover criteria applied for each isolate pair from the same household: I, indistinguishable; D, different; − not possible to compare due the DNA degradation of one isolate during the PFGE restriction.