Table 2. Verified functional C-termini consensus sequences.
| Consensus Sequence | Molecular Function | Description | PubMed Identifier | # Predicted Minimotif Instances3 | # Total Instances4 |
|---|---|---|---|---|---|
| 1x[D/E]x[AVILMFYW]> 2 | Bind | PDZ domain class III binding | 11741967 | 721 | 723 |
| x[AVILMFYW]x[AVILMFYW]>2 | Bind | PDZ domain class II binding | 11741967 | 696 | 702 |
| [KRHQSA][DENQ]EL> | Bind, Traffic | KDEL receptor binding motif | 3545499 | 80 | 81 |
| x[S/T]x[AVILMFYW]>2 | Bind, Traffic | Peroxisomal targeting | 1567655 | 108 | 111 |
| [ST]x[LV]> | Bind | PDZ domain class I binding | 11741967 | 1,432 | 1,441 |
| [STAGCN][KRH][LIVMAFY]> | Bind, Traffic | Peroxisomal targeting | 2901422 | 805 | 839 |
| [WFY]RP[WFY]x(0,6)> | Bind, Traffic | Endoplasmic reticulum (ER) export | 8649374, 12972562 | 114 | 116 |
| C[AVLIFYWM][AVLIFYWM][ACDEFGHIKNPQRSTVWY]> | PTM | Farnesylation | 8702508 2187294 | 104 | 109 |
| C[AVLIFYWM][AVLIFYWM][LM]> | PTM | Geranyl-geranylation | 8702508 2187294 | 70 | 71 |
| Cxxx> | PTM | Farnesylation | 1903399 | 178 | 180 |
| C[GAVLI][GAVLI]x> | PTM | Prenylation | 8702508 | 304 | 306 |
| CxxM> | PTM | Mevalonation | 2686979 | 47 | 48 |
| DEWDx> | Bind | Aldolase binding | 16278221 | 0 | 1 |
| DxE> | Bind, Traffic | COPII binding | 11726510 | 131 | 132 |
| FFxxKKxx> | Bind, Traffic | Arf1 binding motif | 15125774 | 2 | 3 |
| FxxxFxxxF> | Bind, Traffic | ER export | 11331877 | 2 | 3 |
| Kx(0,1)Kx(1,3)> | Bind, Traffic | ER retention | 2120038 | 1,544 | 1,548 |
| S[ST]L> | Bind | PDZ domain class I binding | 11741967 | 84 | 85 |
| SxS> | Bind | Phosphorylation of Smad | 9346966 | 404 | 405 |
| VxPx> | Bind, Traffic | Rod outer segment trafficking | 15728366 | 101 | 102 |
| (V/L)xxSL> | Bind, Traffic | Cell surface expression of Kv1 family K+ channels | 11343973 | 10 | 11 |
| Yxx[AVILMFYW]> | Bind, Traffic | Lysosomal targeting, Dendritic targeting | 9175836, 15689548 | 94 | 98 |
| VMI> | Traffic | ERGIC compartment export | 14517323 | 0 | 1 |
| LxxLxPDExD> | Traffic | Glut4 targeting | 24939910 | 0 | 1 |
| FF> | Bind, Traffic | Endoplasmic Reticulum Export | 9395526 | 78 | 79 |
| HDEL> | Bind, Traffic | Internalization | 2178921 | 12 | 14 |
| KDEL> | Bind, Traffic | Nuclear export, To cell surface & dendrites | 3545499 | 11 | 14 |
| KKx> | Bind, Traffic | To Endoplasmic Reticulum Import | 2120038 | 295 | 296 |
| TOTAL | 7,427 | 7,520 | |||
1”x” indicates any of the twenty amino acids and “> “designates the C-terminal end of a protein [1,46]
2Although a more specific consensus specificity profile for the PDZ domain recognition exists, a more simplified classification was used [28,43,47,48].
3Predicted minimotif instances are matches to consensus sequences that have not yet been experimentally tested.
4Total instances include both predicted and experimentally verified minimotif instances.