Table 2. Ratios of tooth formation in tissue recombination assays.
| Type of recombinant | Culture medium | No. of recombinants | No. of tooth formation | Tooth formation ratio (%) | |
|---|---|---|---|---|---|
| Mesenchyme | Epithelium | ||||
| Dm E14.5 | De E14.5 | --- | 11 | 11 | 100 |
| mDMCs 0h | De E14.5 | M-FBS | 28 | 21 | 78.8 |
| mDMCs 24h | De E14.5 | M-FBS | 28 | 17 | 60.7 |
| mDMCs 48h | De E14.5 | M-FBS | 30 | 0 | 0 |
| mDMCs 120h | De E14.5 | M-FBS | 28 | 0 | 0 |
| mDMCs 192h | De E14.5 | M-FBS | 32 | 0 | 0 |
| mDMCs 0h | De E14.5 | M-KSR | 5 | 5 | 100 |
| mDMCs 24h | De E14.5 | M-KSR | 27 | 14 | 51.9 |
| mDMCs 48h | De E14.5 | M-KSR | 30 | 15 | 50 |
| mDMCs 0h | Se E10.5 | M-KSR | 18 | 7 | 38.8 |
| mDMCs 24h | Se E10.5 | M-KSR | 14 | 4 | 28.6 |
| mDMCs 48h | Se E10.5 | M-KSR | 14 | 3 | 21.4 |
Abbreviations: Dm, dental mesenchyme; De, dental epithelium; Se, second-arch epithelium; mDMCs, mouse dental mesenchymal cells; M-FBS, FBS supplemented medium; M-KSR, KSR supplemented medium.