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. 2016 Apr 6;11(4):e0152405. doi: 10.1371/journal.pone.0152405

Fig 2. Nav1.7 is the major TTX-sensitive Nav channel in small diameter mDRG neurons.

Fig 2

A. RNASeq analysis of Nav channel mRNA from pooled small diameter mouse DRG neurons. B. Structure of PF-05198007 (4-(2-(3-amino-1H-pyrazol-4-yl)-4-(trifluoromethyl)phenoxy)-5-chloro-2-fluoro-N-(thiazol-4-yl)benzenesulfonamide C. Patch clamp data showing concentration-response relationship for PF-05198007 assessed against recombinantly expressed mouse Nav1.7, Nav1.6 and Nav1.1 (IC50, Slope: 5.2 nM, 1.1; 149 nM, 1.5; 174 nM, 0.7 respectively; n = 3–4 per concentration). D. Representative patch clamp current traces of peak sodium current from small diameter mouse DRG neurons in the presence of A-803467 and following concurrent application of PF-05198007 and TTX. E. Representative peak TTX-S current vs time plot before and after 30 nM PF-05198007 and 500 nM TTX. G. Scatter plot of cell capacitance vs Nav1.7/TTX-S ratio (n = 35). Note that in every cell tested, Nav1.7 provided the predominant TTX-S sodium conductance.