Fig 5. Effects of GSK-3α knockdown on the expression of cyclins.

(A) Effect of GSK-3α knockdown on the cell cycle. Indicated cells were starved in serum-free RPMI medium for 24 h and replenished with RPMI supplemented with 10% FBS for another 24 h. Harvested cells were stained with PI to analysis cell cycle by LSRII. (B) Effect of GSK-3α knockdown on the gene expressions related to cell viability or cell cycle including cyclin A2, cyclin B1, cyclin D1, and cyclin E2. The lung cancer cells were transiently transfected with control siRNA, or GSK-3α siRNA (40 nM, each) for 48 h and were followed by western blot analysis.