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. 2016 Mar 16;72(Pt 4):263–268. doi: 10.1107/S2053230X16003794

Table 1. Macromolecule-production information.

Source organism S. pombe
DNA source S. pombe meiotic cDNA library
Forward primer 5′-GATACTAGTATGCGGATTCCTGTTATTCG-3′
Reverse primer 5′-GAACTCGAGTAAACTAATATTTTGTAAGGG-3′
Cloning vector pTYB12-HA (custom-modified plasmid with an HA tag on the C-terminus of the recombinant protein)
Expression vector pTYB12-HA
Expression host E. coli BL21 (DE3)
Complete amino-acid sequence of the construct produced AGHMTSMRIPVIRSPLEIRDTERKGRGVFALEPIPAQTCIEISPVLMFSKEEYEQHGQYTVLNEYTYVWSEGKQGLALGLGSMFNHDRHPNVYWKKDNRNNYISYYTLREIKTNEELCISYGDHLWFEDEASSASRISPNEENEDFPLQNISLLETMYPYDVPDYAAPG

The SpeI site is underlined.

The XhoI site is underlined.