Table 1. Macromolecule-production information.
| Source organism | S. pombe |
| DNA source | S. pombe meiotic cDNA library |
| Forward primer† | 5′-GATACTAGTATGCGGATTCCTGTTATTCG-3′ |
| Reverse primer‡ | 5′-GAACTCGAGTAAACTAATATTTTGTAAGGG-3′ |
| Cloning vector | pTYB12-HA (custom-modified plasmid with an HA tag on the C-terminus of the recombinant protein) |
| Expression vector | pTYB12-HA |
| Expression host | E. coli BL21 (DE3) |
| Complete amino-acid sequence of the construct produced | AGHMTSMRIPVIRSPLEIRDTERKGRGVFALEPIPAQTCIEISPVLMFSKEEYEQHGQYTVLNEYTYVWSEGKQGLALGLGSMFNHDRHPNVYWKKDNRNNYISYYTLREIKTNEELCISYGDHLWFEDEASSASRISPNEENEDFPLQNISLLETMYPYDVPDYAAPG |
The SpeI site is underlined.
The XhoI site is underlined.