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. 2016 Mar 16;72(Pt 4):263–268. doi: 10.1107/S2053230X16003794

Table 2. Crystallization.

Method Hanging drop
Plate type VDX 24-well plates
Temperature (K) 286
Protein concentration (mg ml−1) 6
Buffer composition of protein solution 10 mM Tris buffer pH 7.5
Composition of reservoir solution 0.2 M sodium phosphate dibasic, 20%(w/v) PEG 3350 pH 8.0
Volume and ratio of drop 2.4 µl total volume: 1 µl protein solution, 1 µl reservoir buffer, 0.4 µl additive (0.1 M urea stock, final concentration 16.67 mM)
Volume of reservoir (µl) 500