Table 1. Crystallization.
| Form 1 | Form 2 | |
|---|---|---|
| Method | Sitting-drop vapour diffusion | Sitting-drop vapour diffusion |
| Temperature (K) | 293 | 293 |
| Protein concentration (mg ml−1) | 22.1 | 22.1 |
| Buffer composition of protein solution | 25 mM Tris–HCl pH 7.5, 1 mM DTT | 25 mM Tris–HCl pH 7.5, 1 mM DTT |
| Composition of reservoir solution | 0.1 M bis-tris pH 5.5, 0.2 M lithium sulfate monohydrate, 25%(w/v) PEG 3350 | 0.1 M bis-tris pH 5.5, 0.2 M ammonium sulfate, 25%(w/v) PEG 3350 |
| Volume and ratio of drop | 1 µl:1 µl | 1 µl:1 µl |
| Volume of reservoir (µl) | 100 | 100 |