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. 2016 Mar 16;72(Pt 4):282–287. doi: 10.1107/S2053230X1600279X

Table 1. Crystallization.

Method Hanging-drop vapour diffusion
Plate type 24-well VDX (Hampton Research)
Temperature (K) 293
Protein concentration (mg ml−1) 7–9
Buffer composition of protein solution 30 mM K HEPES pH 7.0, 400 µM BeSO4, 1.6 mM KF, 3 mM MgCl2, 0.5 mM ouabain, 5 mM DTT, 22 mM C12E8
Composition of reservoir solution 14%(w/v) PEG 3350, 100 mM MOPS/N-methyl-D-glucamine pH 7.0, 210 mM MgCl2, 25 mM β-mercaptoethanol
Volume and ratio of drop 2 µl, 1:1 ratio
Volume of reservoir (µl) 400