Table 1. Crystallization.
Method | Hanging-drop vapour diffusion |
Plate type | 24-well VDX (Hampton Research) |
Temperature (K) | 293 |
Protein concentration (mg ml−1) | 7–9 |
Buffer composition of protein solution | 30 mM K HEPES pH 7.0, 400 µM BeSO4, 1.6 mM KF, 3 mM MgCl2, 0.5 mM ouabain, 5 mM DTT, 22 mM C12E8 |
Composition of reservoir solution | 14%(w/v) PEG 3350, 100 mM MOPS/N-methyl-D-glucamine pH 7.0, 210 mM MgCl2, 25 mM β-mercaptoethanol |
Volume and ratio of drop | 2 µl, 1:1 ratio |
Volume of reservoir (µl) | 400 |