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. 2016 Mar 16;72(Pt 4):288–293. doi: 10.1107/S2053230X16003617

Table 2. Crystallization.

Method Vapour diffusion
Plate type Intelli-Plate 96 (Hampton Research)
Temperature (K) 290
Protein concentration (mg ml−1) 80
Buffer composition of protein solution 0.02 M HEPES pH 7.5, 0.15 M NaCl
Composition of reservoir solution 0.05 M calcium chloride dihydrate, 0.1 M bis-tris pH 6.5, 30%(v/v) polyethylene glycol monomethyl ether 550
Volume and ratio of drop 1.0 µl, 1:1
Volume of reservoir (µl) 80