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. 2016 Mar 16;72(Pt 4):294–299. doi: 10.1107/S2053230X16003599

Table 2. Crystallization.

Method Hanging-drop vapour diffusion
Plate type 16-well plates
Temperature (K) 293
Protein concentration (mg ml−1) 15
Buffer composition of protein solution 20 mM Tris–HCl pH 8.0, 150 mM NaCl, 1 mM DTT, 0.2 mM EDTA
Composition of reservoir solution 0.1 M Tris–HCl pH 8.4, 1.25 M potassium sodium tartrate
Volume and ratio of drop 2 µl, 1:1
Volume of reservoir (µl) 500