Table 2. Crystallization.

Method	Sitting-drop vapor diffusion
Plate type	48-well plastic plate
Temperature (K)	293
Protein concentration (mg ml−1)	10
Buffer composition of protein solution	20 mM Tris–HCl pH 8.0, 150 mM NaCl, 5% glycerol, 5 mM DTT
Composition of reservoir solution	35 mM calcium chloride dehydrate, 70 mM MES monohydrate pH 6.0, 30 mM Tris pH 8.5, 31.5% PEG 200, 6% ethanol
Volume and ratio of drop	1 µl protein solution plus 1 µl reservoir solution
Volume of reservoir (µl)	100