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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: Behav Brain Res. 2015 Dec 14;301:1–9. doi: 10.1016/j.bbr.2015.12.012

Figure 5. Effect of high fat diet and metformin treatment on neurotrophic factor expression in brain.

Figure 5

(A) BDNF mRNA levels. (B) Protein levels of preBDNF and mature BDNF. (C) NGF mRNA levels (D) NGF protein levels, (E) NTF3 mRNA levels. (F) NTF3 Protein levels. * = significantly different from C and HF, p < 0.001. All mRNA levels were normalized to GAPDH and protein levels were normalized to β-actin protein or ponsceau S. Error bar = ± standard error of mean.