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. 2015 Dec 28;2(2):189–209. doi: 10.1016/j.jcmgh.2015.11.010

Figure 5.

Figure 5

Apoptotic cell death was not increased in intestine lacking GATA4. Immunofluorescent staining with an antibody against cleaved caspase 3 (green) was used to assess apoptotic cell death in intestine from control and Gata4loxP/-ShhCre embryos at E12.5, E14.5, and E16.5 (n = 3 controls and mutants per stage). E-cadherin staining (red) was used to mark the epithelium. 4',6-diamidino-2-phenylindole (DAPI) (blue) was used to mark nuclei. No differences were observed between groups. Scale bars: 50 μm.