Table 1.
Vitamin D analogs tested for potential interference
| Vitamin D metabolite or structural analog | Relative retention time a | m/z |
|---|---|---|
| 3-epi-25(OH)D3 | 1.11 | 383→365 |
| 3-epi-25(OH)D2 | 1.12 | 395→377 |
| 7α-hydroxy-4-cholestene-3-one | 2.78 | 383→365 |
| 1α-hydroxyvitamin D3 | 2.25 | 383→365 |
| 4β-hydroxycholesterol | 5.07 | 385→367 |
| 27-hydroxycholesterol | 1.83 | 385→367 |
| 24(S)-hydroxycholesterol | 1.47 | 385→367 |
| 25-hydroxycholesterol | 1.62 | 385→367 |
Relative retention time is calculated relative to 25(OH)D3 (m/z 383→365) or 25(OH)D2 (m/z 395→377)