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. 2016 Jan 4;7(3):2159–2174. doi: 10.18632/oncotarget.6818

Figure 4. miR145 promotes gene expression of differentiation markers in KRASmut colon cancer cells and inhibits tumorigenesis.

Figure 4

A. Relative U6 and miR145 levels in KRASmut SW48 cells expressing lenti-pCCL-Scramble and lenti-pCCL-miR145 as measured by quantitative RT-PCR and normalized to parental SW48 cells. B. Relative gene expression levels of differentiation marker genes in KRASmut SW48 cells expressing lenti-pCCL-Scramble or lenti-pCCL-miR145 as quantified by RT-PCR and normalized to parental KRASmut SW48 cells. Brachyury (T), transcription factor GATA4, (GATA4), Notch homolog 1 translocation-associated (Notch1), E-cadherin (CDH1). C. Colony Growth of KRASmut SW48 cells expressing lenti-pCCL-Scramble or lenti-pCCL-miR145 in soft agar assays with representative images (right). D. Tumor xenografts of KRASmut SW48 cells expressing lenti-pCCL-Scramble and lenti-pCCL-miR145 with representative tumor images (upper). (***, p < 0.001). Quantitative data were averaged from n ≥ 3 independent experiments.