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. 2016 Jan 6;7(3):2269–2283. doi: 10.18632/oncotarget.6825

Figure 3. Complex formation between Plk1 and BRCA1 is reduced upon CDK1 inhibition.

Figure 3

A. BRCA1 and Plk1 co-immunoprecipitate in cellulo. Whole-cell extracts from asynchronous HeLa cells were incubated with anti-BRCA1 or -Plk1 antibody. Immune complexes were recovered with protein A-sepharose beads (IP) and analyzed by immunoblotting (IB) with anti-BRCA1 and anti-Plk1 antibodies. IP Φ, control IP. B. Complex formation between BRCA1 and Plk1 is reduced upon CDK1 inhibition. HeLa cells were left untreated (NT) or were treated with RO3306 for 4 h, or with calicheamicin (CLM) for 1 hour before co-immunoprecipitation. Equal quantities of whole-cell extracts were then incubated with anti-BRCA1 antibody. Immune complexes were recovered with protein A-sepharose beads (IP) and analyzed by immunoblotting (IB) with anti-BRCA1 and -Plk1 antibodies. IP Φ, control IP.