Table 1. Summary of in vitro MIA, SRB & PI data for CCT245737.
| Cells | MIA (μM) |
GI50 (μM) |
Genotoxic Agent |
Combination GI50 (μM) |
Potentiation Index (PI) |
|---|---|---|---|---|---|
| HT29 | 0.030±0.012 (n = 6) | 0.70±0.29 (n = 6) | SN38 | 0.39±0.061 (n = 3) | 1.8±0.31* (n = 3) |
| Gem | 0.09±0.023 (n = 6) | 7.9±2.1*** (n = 6) | |||
| SW620 | 0.22±0.047 (n = 3) | 5.4±1.4 (n = 5) | SN38 | 1.8±0.57 (n = 4) | 3.1±1.5* (n = 4) |
| Gem | 0.33±0.064 (n = 7) | 17±3.4*** (n = 7) | |||
| MiaPaCa-2 | 0.063±0.011 (n = 3) | 1.3±0.40 (n = 3) | SN38 | 0.43±0.062 (n = 3) | 3.1±0.43* (n = 3) |
| Gem | 0.066±0.025 (n = 3) | 23±9.6 (n = 3) | |||
| Calu6 | 0.084±0.0070 (n = 3) | 0.41±0.059 (n = 3) | SN38 | 0.32±0.021 (n = 3) | 1.3±0.084* (n = 3) |
| Gem | 0.046±0.0069 (n = 3) | 9.1±1.5* (n = 3) |
Mitosis induction assay (MIA IC50) results and cytotoxicity (GI50) values were determined as described in Material and Methods. Potentiation index (PI) is the ratio of GI50 / Combination GI50. Potentiation index (PI) values.1 indicate potentiation of the genotoxic drug. Values are mean±SD of n independent determinations.
*
Statistical significance: P < 0.05
**
P < 0.01
***
P < 0.001.