Figure 3. Suppressing TREM2 expression induced S phase arrest and apoptosis in glioma cells.

U87 and U373 cells were transfected with indicated siRNA and collected 48 hours later cells. A., B. Cell cycle profile was analyzed using flow cytometry. C., D. Cell apoptosis was analyzed by Annexin V/PI staining. Data were based on at least 3 independent experiments, and shown as the mean ± S.D. (*P < 0.05, ** P < 0.01, ***P < 0.001 compared with NC).