Figure 5. Growth suppression of established s.c. xenografts by CARgpc3 T cells.

(A), (C) The tumor volumes and weights were quantified. (B), (D) CARgpc3 T cells could effectively remove the GPC3-positive LSCC cells. Tumors were collected from mice with NCI-H520-GPC3 or SK-MES-1-GPC3 subcutaneous xenografts treated with CAR T cells or saline. Formalin-fixed, paraffin-embedded tumor sections were consecutively cut and stained for human GPC3 expression (brown) [The images were obtained with a microscope (BX41, Olympus, PA) and camera (DP70) under × 200 magnification. The scale bar is equal to 200 μm]; in addition, a western blot analysis of the expression of GPC3 in NCI-H520-GPC3 and SK-MES-1-GPC3 xenografts is shown.