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. 2015 Nov 11;7(3):2532–2544. doi: 10.18632/oncotarget.6304

Figure 4. Simvastatin-induced anti-tumoral effects of apoptosis induction, proliferation inhibition and deactivation of PI3K/Akt and MAPK/ERK pathway were blocked by mevalonate, FPP and GGPP.

Figure 4

MCF-7, T47D, MDA-MD-231 and BT-549 were pre-treated with mevalonate (200μM), FPP (10μM) and GGPP (20μM) for 1 hour respectively, followed by incubation with or without simvastatin (20μM for MCF-7 and T47D; 0.8μM for MDA-MB-231 and 10μM for BT-549) for 48 hours. Figure 4A-D. The activity of caspase-3/7 was determined by caspase-3/7 assay. Figure 4E-H. Cell viability was determined by MTS assay. Figure 4I-L. The levels of phosphorylated and total proteins of Akt and ERK1/2 were examined by Western blots analysis. * P < 0.05, ** P < 0.01 and ***P < 0.001 versus cells treated with simvastatin.