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. 2015 Dec 9;7(3):2660–2671. doi: 10.18632/oncotarget.6545

Figure 2. MiR-497 inhibits cell proliferation, migration and invasion.

Figure 2

A. Relative expression levels of miR-497 in SW1116/miR-497 and SW1116/miR-NC stable cell lines were confirmed by Taqman RT-qPCR. B. SW1116/miR-497 and SW1116/miR-NC cells were plated 2000 cells per well in 96-well plates, and cell proliferation was determined using Cell Counting Kit-8 (CCK-8) to detect the absorbance at 450 nm every day. C. SW1116/miR-497 and SW1116/miR-NC cells were cultured to 90% confluence. A sterile 200 μl pipette tip was used to scratch the cells to form a wound. The wound gaps were photographed and measured. D. Matrigel invasion assay of SW1116/miR-497 and SW1116/miR-NC cells were performed as previously described. Data represent mean ± SD from 3 replicates. * indicates significant difference at p < 0.05 when compared to the miR-NC control; ** indicates significant difference at p < 0.01 when compared to the miR-NC control.