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. 2015 Dec 9;7(3):2660–2671. doi: 10.18632/oncotarget.6545

Figure 4. Overexpression of KSR1 reverses miR-497-mediated suppression of cell proliferation, migration and invasion.

Figure 4

A. SW1116 cells were co-tranfected with miR-497 or miR-NC, and with empty vector or KSR1 cDNA without 3′-UTR region. After 72 h culture, immunoblotting assay was performed as described above. B. Cells were treated as above, and cell proliferation assay was performed using CCK-8 kit. C. Cells were transfected as described above. A sterile 200 μl pipette tip was used to scratch the cells to form a wound. The wound gaps were photographed and measured. D. miR-497 overexpression decreased invasion of SW1116 cells, and overexpression of KSR1 reverses the inhibitory effects of miR-497. The cells treated as above were subjected to a Matrigel invasion assay. Data represent mean ± SD of three replicates. * indicates significant difference at p < 0.05 compared to miR-NC control; ** indicates significant difference at p < 0.01 compared to miR-NC control. # indicates significant difference at p < 0.05 compared to miR-497 and KSR1 overexpression; ## indicates significant difference at p < 0.01 compared to miR-497 and KSR1 overexpression.