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. 2015 Nov 27;7(3):2765–2779. doi: 10.18632/oncotarget.6409

Figure 2. The PARG inhibitor ethacridine lactate sensitizes AML cell lines to ibrutinib.

Figure 2

(A) Ibrutinib was co-treated with 240 drugs in TEX and OCI-AML2 cells for 72 h. Growth and viability was determined with the SRB assay and synergy was calculated using the EOBA formula as described in the methods. Compounds were ranked in order of increasing average positive EOBA score. (B, C) TEX and OCI-AML2 cells were combination-treated with ibrutinib and ethacridine for 72 h and cell growth and viability relative to untreated cells was determined by Alamar Blue. (B) Data represent mean growth and viability ± SD from a representative experiment performed in triplicate. (C) EOBA synergy scores (shown) were calculated for each of the combinations tested. EOBA values > 0.1 (lightest grey) denote a synergistic combination, while values > 0.5 (darkest grey) denote a strongly synergistic combination. Data represent mean EOBA scores from a representative experiment performed in triplicate.