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. 2015 Dec 20;7(3):2878–2888. doi: 10.18632/oncotarget.6694

Figure 3. Up-regulation of TLE4 promotes cell proliferation and invasion activity of CRC cells.

Figure 3

(A) Overexpression of TLE4 in SW480 and HT29 cells analyzed by Western blotting. α-Tubulin was used as a loading control. (B and C) Overexpression of TLE4 promotes SW480 and HT29 cell proliferation in MTT assays (B) and colony formation assays (C). (D) Ectopic expression of TLE4 promotes anchorage independent growth ability of SW480 and HT29 cells as determined by Soft agar assays. Colonies containing more than 50 cells were scored. Each bar represents the mean ± SD of 3 independent experiments. (E) The invasive abilities of CRC cells evaluated using the Matrigel-coated Boyden chamber invasion assay. Each bar represents the mean ± SD of three independent experiments (F) Xenograft model was built by injected SW480/Vector and SW480/TLE4 cells in nude mice (n = 5/group). Tumor volumes were measured on the indicated days. Data points are the mean tumor volumes ± SD. (G) The sections of tumor were under H & E staining or subjected to IHC staining using an antibody against Ki-67. (H) The expression of TLE4 in xenograft tumor was analyzed with Real-time PCR. Error bar represents the mean ± SD.