Figure 4. Glucose-6-phosphate dehydrogenase inhibition with siRNA reduces proliferation of glycolytic cancer cells.

MDA-MB-231 cancer cells were transfected with siRNAs targeting G6PD (siG6PD) or non-targeting siRNAs (siCTL). 48 hours after transfection, cells were subjected to A. immunoblot analysis using Hsp90 as loading control, B. NADP⁺ and NADPH levels quantification and C. DNA synthesis measurement. Two-sided t test. *p < 0.05, ***p < 0.001. Results are expressed as means ± SEM.