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. 2015 Nov 26;7(3):2921–2935. doi: 10.18632/oncotarget.6404

Figure 4. Specific knockdown of erbB3, but not IGF-1R, markedly enhances lapatinib-induced apoptosis in trastzumab-resistant breast cancer cells.

Figure 4

SKBR3-Pool2 and BT474-HR20 cells infected with lentivirus containing either ConshRNA or erbB3/IGF-1R shRNA (erbB3shRNA or IGF-1RshRNA). The cells were then untreated or treated with lapatinib (0.1 μmol/L) for 24 hr and subjected to the following experiments. A & B. Western blot analyses of erbB3, IGF-1R, PARP (F-PARP, full length PARP; C-PARP, cleaved PARP), caspase-8 (F-Casp-8, full length caspase-8; C-Casp-8, cleaved caspase-8), caspase-3 (F-Casp-3, full length caspase-3; C-Casp-3, cleaved caspase-3), or β-actin (A); or a specific apoptosis ELISA (B). Bars, SD. C. Live/dead cell staining. Cells freshly stained with Live/Dead Imaging kit. Red indicated the dead cells (white arrow).