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. 2015 Dec 20;7(3):2951–2967. doi: 10.18632/oncotarget.6696

Figure 7. LPAR1 or LPAR3 is effectively knocked down in SKHep1 cells in vitro using stable shRNA transfection.

Figure 7

(A) qRT-PCR mRNA and representative Western blot protein analysis of LPAR1 expression in non-transfected SKHep1 cells or SKHep1 clones following stable transfection with an off-target scrambled sequence (Scr) or an shRNA against LPAR1 (shLPAR1 Cl8 and Cl11). *p < 0.05 Scr vs. shLPAR1. (B) qRT-PCR mRNA and representative Western blot protein analysis of LPAR3 expression in non-transfected SKHep1 cells or SKHep1 clones following stable transfection with an off-target scrambled sequence (Scr) or an shRNA against LPAR3 (shLPAR3 Cl8 and Cl9). *p < 0.05 Scr vs. shLPAR3.