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. 2015 Nov 27;7(3):3084–3097. doi: 10.18632/oncotarget.6413

Figure 2. DNA methylation-associated transcriptional silencing of SLFN11.

Figure 2

A. Bisulfite genomic sequencing of SLFN11 promoter CpG island. CpG dinucleotides are represented as short vertical lines. At least eight single clones are shown for each sample. Presence of a methylated or unmethylated cytosine is indicated by a red or green square, respectively. MDA-MB-231, MCF-7, HCT-15, and HCT-116 show dense CpG island methylation. B. Expression levels of the SLFN11 transcript and protein determined by real-time reverse transcription-PCR and western blot, respectively. C. SLFN11 (green label) and DAPI (blue label) immunofluorescence in the studied cancer cell lines. D. The expression of SLFN11 RNA transcript and protein was restored in the methylated HCT15 and MDA-MB-231 cells by treatment with the demethylating drug 5-aza-2-deoxycytidine (AZA). E. Genetic disruption of the two major DNA methyltransferases DNMT1 and DNMT3B (in DKO cells) also restored SLFN11 RNA and protein expression in HCT-116 cells. Data are summarized as the mean ± s.e.m. of three biological replicates. ***p < 0.001,