Figure 8. RU cells from patient samples can also convert to RR cells upon H₂O₂ treatment.

Tumor cells from 2 primary patient breast tumor samples were infected with the lentiviral SRR2 reporter and RU cells were sorted using flow cytometry. Purified RU cells were treated with H₂O₂ (0.5 mM) for 6 hours and analyzed for GFP and luciferase activity. A. Flow cytometry results of a representative sample are illustrated, and we observed an increase in the proportion of GFP-positive tumor cells after H₂O₂ treatment. B. The luciferase activity of both tumor samples treated H₂O₂ was significantly higher than that of the negative (untreated) controls.