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. 2015 Dec 11;7(3):3233–3244. doi: 10.18632/oncotarget.6564

Figure 5. Induction of apoptosis by FTY720 in ACC cells.

Figure 5

Flow cytometry results of annexin V-PI stained ACC cells after exposure to different concentration of FTY720 for 24 h. (A) An increase in apoptotic cells following treatment with FTY720 is shown in H295R cells; (B) An increase in apoptotic cells following treatment with FTY720 is shown in SW13 cells; (C) The combination of FTY720 and mitotane had a significant influence on the apoptotic rate in SW13 cells, but there is no effect of the combination of FTY720 and mitotane on the apoptosis of H295R; (D) The flow cytometry profile represents SW13 cells with annexin V-FITC staining on the x-axis and PI on the y-axis. Dual staining of cells with annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) FITC and PI enabled categorization of cells into 4 regions. Region Q1 shows dead cells, Q2 shows late apoptotic cells, Q3 shows early apoptotic cells, and Q4 shows living cells. Q2 and Q3 are collectively called apoptotic cells.