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. 2015 Oct 29;7(3):3599–3613. doi: 10.18632/oncotarget.6120

Figure 6. NOTCH1 acts as a tumor suppressor gene in ESCC.

Figure 6

A. Knockdown of endogenous NOTCH1 in KYSE150 and KYSE140 cells and subsequent over-expression of NOTCH1 wild-type or W745X mutant were demonstrated by Western Blotting. GAPDH was used as loading control. B. Growth assay shows promoting of proliferation by knockdown NOTCH1 in KYSE150 and KYSE140 cells. Overexpression of NOTCH1 wild-type but not W745X mutant significantly suppressed cell proliferation. Data are mean ± SD; each experiment was performed in triplicate. **P < 0.01; *P < 0.05. C.-D. Knockdown of NOTCH1 or subsequent over-expression of NOTCH1 wild-type or W745X mutant does not affect migration/invasion of KYSE150 and KYSE140 cells. The data are mean ± SD; each experiment was performed in triplicate.