Table 1. Summary of clinical characteristics of ESCC patients with NOTCH1 and PIK3CA mutations in cohort #1.

Clinical, epidemiological or pathological feature		NOTCH1				PIK3CA
		Total(N)	Mutant	Wild-type	P value	Total(N)	Mutant	Wild-type	P value
All cases		104	22	82		104	17	88
Age	<60	50	8	42	0.302	50	5	45	0.199
	60-69	40	12	28		40	7	33
	≥70	14	2	12		14	4	10
Sex	Male	104				104
	Female	0				0
Tobacco use	Yes	96	19	77	0.467	96	15	81	1
	No	8	3	5		8	1	7
Alcohol consumption	Yes	63	11	52	0.253	62	9	53	0.766
	No	41	11	30		42	7	35
Family cancer history	Yes	17	5	12	0.557	17	2	15	1
	No	87	17	70		87	14	73
Tumor location	Upper thoracic	4	0	4	0.027	4	3	1	0.006
	Middle thoracic	42	4	38		42	3	39
	Lower thoracic	57	17	40		57	10	47
Histological grade	Grade 1	59	20	39	0.001	59	10	49	0.328
	Grade 2	33	0	33		33	6	27
	Grade 3	12	2	10		12	0	12
Pathologic Stage	I	60	18	42	0.011	51	8	43	0.933
	II
	III	44	4	40		53	8	45
Pathologic T stage	T1	4	3	1	0.004	4	0	4	0.604
	T2	23	6	17		23	5	18
	T3	72	13	59		72	10	62
	T4	5	0	5		5	1	4
Pathologic N stage	0	54	18	36	0.002	54	9	45	0.706
	1	50	4	46		50	7	43
Local recurrence	Yes	39	12	27	0.063	39	3	36	0.092
	No	56	10	46		65	13	52
Chemotherapy treatment	Failure	39	11	28	0.253	39	3	36	0.023
	effective	31	5	26		31	10	21
	No treatment	24	4	20		24	3	21
	missing	10	1	9		10	0	10
Prognosis (Log-rank Mantel-Cox test)	Dead	45	8	37	0.518	45	4	41	0.048
	Survival	41	11	30		41	12	29
	Missing	18	3	15		18	0	18
	Median (months)		60	80			47.5	24

Note: The ESCC cases collected for this study were staged using the NCCN guidelines.